Milk Inducting in Non-Lactating Ewes (OvisAries) in Off-Breeding Season

Authors

  • Sayed Qadir Danishiar Kabul University, Department of Biotechnology, Faculty of Agriculture, Kabul, Afghanistan
  • Jahid Zabuli Kabul University, Department of Clinic, Faculty of Veterinary Sciences, Kabul, Afghanistan
  • Habibullah Rahamani Kabul University, Department of Animal Sciences, Faculty of Agriculture, Kabul, Afghanistan
  • Mohammadullah Arghandiwal Kabul University, Department of Animal Sciences, Faculty of Agriculture, Kabul, Afghanistan

DOI:

https://doi.org/10.62810/jnsr.v4i3.566

Keywords:

Estrogen, Milk production, Non-lactating ewes, Progesterone

Abstract

Turkish ewes (Ovis aries) milk production is limited by seasonal breeding patterns, resulting in reduced milk availability during the off-breeding season when ewes are neither pregnant nor lactating. This study aimed to evaluate a hormonal protocol for inducing lactation and assess mammary gland development, milk production and composition, and physiological responses. The study was conducted at the Faculty of Agriculture, Kabul University, Afghanistan, from 23 August–14 September 2025. Eight dry, non-pregnant Turkish ewes received estradiol benzoate and progesterone during Days 1–7, dexamethasone, and udder massage during Days 1–10. Udder length, rectal temperature, milk yield, milk composition, and behavioral responses were evaluated. We analyzed data using IBM SPSS Statistics version 20. Data were summarized as mean ± standard deviation, while one-way ANOVA and repeated-measures ANOVA assessed differences, with LSD tests for multiple comparisons. Udder length increased from 2.87 ± 1.72 cm on Day 1 to 6.85 ± 2.19 cm on Day 23 (P < 0.001). Milk yield ranged from 10 to 130 mL per 24 h, and protein differed significantly from the reference value (P = 0.002). Overall, the findings support increased milk production and improved farm economics.

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References

Aguirre-Flores, V., Orihuela, A., & Vázquez-Rosales, R. (2018). Milk and blood progesterone concentration in ewes (Ovis aries) under different physiological states and progestogen treatment. Journal of Applied Animal Research, 46(1), 1313–1316. https://doi.org/10.1080/09712119.2018.1503962 DOI: https://doi.org/10.1080/09712119.2018.1503962

Baumgard, L. H., Collier, R. J., & Bauman, D. E. (2017). A 100-Year Review: Regulation of nutrient partitioning to support lactation. Journal of Dairy Science, 100(12), 10353–10366. https://doi.org/10.3168/jds.2017-13242 DOI: https://doi.org/10.3168/jds.2017-13242

Berryhill, G. E., Trott, J. F., & Hovey, R. C. (2016). Mammary gland development-It’s not just about estrogen. Journal of Dairy Science, 99(1), 875–883. https://doi.org/10.3168/jds.2015-10105 DOI: https://doi.org/10.3168/jds.2015-10105

Choudhary, R. K., Pathak, D., Choudhary, S., & Verma, R. (2018). Immunolocalization of estrogen alpha and progesterone beta receptors in goat mammary gland. Indian Journal of Animal Sciences, 88(4), 420–427. https://doi.org/10.56093/ijans.v88i4.78803 DOI: https://doi.org/10.56093/ijans.v88i4.78803

Confuorti, C., Jaramillo, M., & Plante, I. (2024). Hormonal regulation of miRNA during mammary gland development. Biology Open, 13(6), 1–19. https://doi.org/10.1242/bio.060308 DOI: https://doi.org/10.1242/bio.060308

Do, D. N., Li, R., Dudemaine, P. L., & Ibeagha-Awemu, E. M. (2017). MicroRNA roles in signalling during lactation: An insight from differential expression, time course and pathway analyses of deep sequence data. Scientific Reports, 7(October 2016), 1–19. https://doi.org/10.1038/srep44605 DOI: https://doi.org/10.1038/srep44605

Freitas, P. R. C., Coelho, S. G., Rabelo, E., Lana, Â. M. Q., Artunduaga, M. A. T., & Saturnino, H. M. (2010). Artificial induction of lactation in cattle. Revista Brasileira de Zootecnia, 39(10), 2268–2272. https://doi.org/10.1590/s1516-35982010001000024 DOI: https://doi.org/10.1590/S1516-35982010001000024

Hooper, H. B., Silva, P. dos S., de Oliveira, S. A., Meringhe, G. K. F., Lacasse, P., & Negrão, J. A. (2020). Effect of heat stress in late gestation on subsequent lactation performance and mammary cell gene expression of Saanen goats. Journal of Dairy Science, 103(2), 1982–1992. https://doi.org/10.3168/jds.2019-16734 DOI: https://doi.org/10.3168/jds.2019-16734

Itana, D. D., & Duguma, A. (2021). The Role and Impacts of Growth Hormones in Maximizing Animal Production- A review. Turkish Journal of Agriculture - Food Science and Technology, 9(6), 975–981. https://doi.org/10.24925/turjaf.v9i6.975-981.3852 DOI: https://doi.org/10.24925/turjaf.v9i6.975-981.3852

Khan, M. A., Bughio, S., Buriro, R., Arain, M. B., Soomro, S. A., Mughal, G. A., & Lanjar, Z. (2022). Residual Potential of Dexamethasone and its Effect on Goat Milk. International Journal of Agriculture and Biology, 27(1), 28–33. https://doi.org/10.17957/IJAB/15.1895 DOI: https://doi.org/10.17957/IJAB/15.1895

Lacasse, P., Ollier, S., Lollivier, V., & Boutinaud, M. (2016). New insights into the importance of prolactin in dairy ruminants. Journal of Dairy Science, 99(1), 864–874. https://doi.org/10.3168/jds.2015-10035 DOI: https://doi.org/10.3168/jds.2015-10035

Li, S. N., Tang, S. H., He, Q., Hu, J. X., & Zheng, J. (2020). In vitro antioxidant and angiotensin-converting enzyme inhibitory activity of fermented milk with different culture combinations. Journal of Dairy Science, 103(2), 1120–1130. https://doi.org/10.3168/jds.2019-17165 DOI: https://doi.org/10.3168/jds.2019-17165

Lu, Y., Govindasamy-Lucey, S., & Lucey, J. A. (2016). Angiotensin-I-converting enzyme-inhibitory peptides in commercial Wisconsin Cheddar cheeses of different ages. Journal of Dairy Science, 99(1), 41–52. https://doi.org/10.3168/jds.2015-9569 DOI: https://doi.org/10.3168/jds.2015-9569

Luz, G. B., Maffi, A. S., Xavier, E. G., Correa, M. N., Gasperin, B. G., & Brauner, C. C. (2020). Induction of lactation in dairy heifers: Milk production, inflammatory and metabolic aspects. Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, 72(2), 371–378. https://doi.org/10.1590/1678-4162-11246 DOI: https://doi.org/10.1590/1678-4162-11246

Paiano, R. B., Lahr, F. C., Poit, D. A. S., Costa, A. G. B. V. B., Birgel, D. B., & Birgel Junior, E. H. (2018). Biochemical profile in dairy cows with artificial induction of lactation. Pesquisa Veterinaria Brasileira, 38(12), 2289–2292. https://doi.org/10.1590/1678-5150-PVB-5951 DOI: https://doi.org/10.1590/1678-5150-pvb-5951

Pires, J. A. A., Stumpf, L. F., Soutullo, I. D., Pescara, J. B., Stocks, S. E., & Grummer, R. R. (2016). Effects of abomasal infusion of nicotinic acid on responses to glucose and β-agonist challenges in underfed lactating cows. Journal of Dairy Science, 99(3), 2297–2307. https://doi.org/10.3168/jds.2015-10308 DOI: https://doi.org/10.3168/jds.2015-10308

Radavelli, W. M., Campigotto, G., Machado, G., Bottari, N. B., Bochi, G., Moresco, R. N., Morsch, V. M., Schetinger, M. R. C., Bianchi, A., Baldissera, M. D., Ferreira, R., & da Silva, A. S. (2016). Effect of lactation induction on milk production and composition, oxidative and antioxidant status, and biochemical variables. Comparative Clinical Pathology, 25(3), 639–648. https://doi.org/10.1007/s00580-016-2243-z DOI: https://doi.org/10.1007/s00580-016-2243-z

Ramgattie, R., Siew, N., Diptee, M., Stoute, V., & Knights, M. (2014). Effect of mammary stimulation on dairy cows and heifers exposed to a lactation induction protocol. Open Journal of Animal Sciences, 04(01), 1–12. https://doi.org/10.4236/ojas.2014.41001 DOI: https://doi.org/10.4236/ojas.2014.41001

Ramirez Ramirez, H. A., Harvatine, K. J., & Kononoff, P. J. (2016). Short communication: Forage particle size and fat intake affect rumen passage, the fatty acid profile of milk, and milk fat production in dairy cows consuming dried distillers grains with solubles. Journal of Dairy Science, 99(1), 392–398. https://doi.org/10.3168/jds.2015-10006 DOI: https://doi.org/10.3168/jds.2015-10006

Salmon-Legagneur, M., Perrin, J., Tabardel, N., Barrier, C., Mellare, M., Ducrocq, L., Kotevski, A., Poggi, L., Coutelier, H., Pezé-Heidsieck, E., Benachi, A., Wartelle, T., Perrin, A., Ayoubi, J. M., Rigourd, V., & Neveu, G. (2026). Impact of lactation stage, maternal age, and parity on mammary epithelial cell populations in fresh milk. Scientific Reports, 16(1), 0–41. https://doi.org/10.1038/s41598-025-33232-y DOI: https://doi.org/10.1038/s41598-025-33232-y

Sawyer, G. J., Fulkerson, W. J., Martin, G. B., & Gow, C. (1986). Artificial Induction of Lactation in Cattle: Initiation of Lactation and Estrogen and Progesterone Concentrations in Milk. Journal of Dairy Science, 69(6), 1536–1544. https://doi.org/10.3168/jds.S0022-0302(86)80570-7 DOI: https://doi.org/10.3168/jds.S0022-0302(86)80570-7

Skliarov, P., Pérez, C., Petrusha, V., Fedorenko, S., & Bilyi, D. (2021). Induction and synchronization of oestrus in sheep and goats. Journal of Central European Agriculture, 22(1), 39–53. https://doi.org/10.5513/JCEA01/22.1.2939 DOI: https://doi.org/10.5513/JCEA01/22.1.2939

Toledo, I. M., Zhao, X., & Lacasse, P. (2020). Effects of milking frequency and domperidone injections on milk production and prolactin signaling in the mammary gland of dairy cows. Journal of Dairy Science, 103(2), 1969–1981. https://doi.org/10.3168/jds.2019-17330 DOI: https://doi.org/10.3168/jds.2019-17330

Vailati-Riboni, M., Kanwal, M., Bulgari, O., Meier, S., Priest, N. V., Burke, C. R., Kay, J. K., McDougall, S., Mitchell, M. D., Walker, C. G., Crookenden, M., Heiser, A., Roche, J. R., & Loor, J. J. (2016). Body condition score and plane of nutrition prepartum affect adipose tissue transcriptome regulators of metabolism and inflammation in grazing dairy cows during the transition period. Journal of Dairy Science, 99(1), 758–770. https://doi.org/10.3168/jds.2015-10046 DOI: https://doi.org/10.3168/jds.2015-10046

Yao, D. W., Ma, J., Yang, C. L., Chen, L. L., He, Q. Y., Coleman, D. N., Wang, T. Z., Jiang, X. L., Luo, J., Ma, Y., & Loor, J. J. (2021). Phosphatase and tensin homolog (PTEN) suppresses triacylglycerol accumulation and monounsaturated fatty acid synthesis in goat mammary epithelial cells. Journal of Dairy Science, 104(6), 7283–7294. https://doi.org/10.3168/jds.2020-18784 DOI: https://doi.org/10.3168/jds.2020-18784

Yart, L., Lollivier, V., Marnet, P. G., & Dessauge, F. (2014). Role of ovarian secretions in mammary gland development and function in ruminants. Animal, 8(1), 72–85. https://doi.org/10.1017/S1751731113001638 DOI: https://doi.org/10.1017/S1751731113001638

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Published

2026-10-01

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Section

Veterinary and Animal Sciences

How to Cite

Danishiar, S. Q., Zabuli, J., Rahamani, H., & Arghandiwal, M. (2026). Milk Inducting in Non-Lactating Ewes (OvisAries) in Off-Breeding Season. Journal of Natural Science Review, 4(3), 1071-1088. https://doi.org/10.62810/jnsr.v4i3.566