Genetic Transformation of Tomato (Solanum Lycopersicum. L) Using Cry1Ac Gene to Impart Resistance Against Fruit Borer

Rizwan Rashid; Zahedullah Zahed

doi:10.62810/jnsr.v2iSpecial.Issue.136

Authors

Rizwan Rashid University of Agricultural Sciences &Technology of Kashmir, Shalimar, Srinagar
Zahedullah Zahed Wardak Institute of Higher Education, Maidan Wardak, Afghanistan

DOI:

https://doi.org/10.62810/jnsr.v2iSpecial.Issue.136

Keywords:

Cry1Ac, GUS Assay, Lycopersicon Esculentum, Tomato genetic transformation

Abstract

Particle gun-mediated genetic transformation was used to produce transgenic plants of tomato (Solanumlycopersicum L.) resistant to fruit borer. Cotyledon and leaf explants from invitro tomato seedlings of variety ‘Punjab Upma’ with Cry1Ac gene construct (pGEM-4Z) were driven by Ubi promoter and NOS terminator, and GUS gene construct (pWRG 1515) were driven by CaMV 35S promoter and NOS terminator. The constructs were maintained as plasmids in E. coli strain JM109. These results signify the successful introduction of the Cry1Ac gene into tomato plants. Further PCR analysis for the Cry1Ac gene of three putative transgenic plants were performed, but no amplification was observed.

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