Studies on Aflatoxin-Producing Fungi in Stored Maize (Zea mays L.) and the Use of Bentonite Clay in Reducing their Toxin Levels

Ahmadu  Umaru; Aliyu Isa; Rabia Ayoubi

doi:10.62810/jnsr.v2iSpecial.Issue.124

Authors

Ahmadu Umaru Agriculture,Maiduguri, Nigeria University
Aliyu Isa Department of Science Laboratory Technology, Ramat Polytechnic, Maiduguri, Nigeria
Rabia Ayoubi Department of Pharmacognosy, Faculty of Pharmacy, Kabul University, Kabul, Afghanistan

DOI:

https://doi.org/10.62810/jnsr.v2iSpecial.Issue.124

Keywords:

Bentonite Clay, AgraQuant, Aflatoxins, ELISA, MEGA, NCBI

Abstract

Aflatoxins are a group of mycotoxins produced by Aspergillus fungi that are both toxic and carcinogenic to animals and humans; however, studies on their removal by natural substances have had great success, and bentonite was seen as a possible remedy in this aspect. The study on aflatoxin-producing fungi in stored Maize (Zea mays L.) was conducted on stored maize collected from Muna Market, Maiduguri, based on their year of harvest (2015, 2016, 2017, and 2018). Out of which a total of 49 isolates consisting of Aspergillus spp, Talaromyces islandicus, and Scopulariopsis candida were obtained, these include Aspergillus niger 38.78%, A. flavus 12.24%, A. oryzae 12.24%, A. fumigatus 10.20%, A. parvisclerotigenus 6.12%, A. aflatoxiformans 4.08%, Scopulariopsis candida 14.29%, and Talaromyces islandicus 2.04%. The molecular assay confirms the identity of the isolates amplified using universal ITS primers with 100% query and identity, except sample A (A. aflatoxiformans 087-A2) has 99.62% identity compared with NCBI library. The total aflatoxin profile of the stored maize collected for the 2015 harvest year was 100 ppb before treatment and reduced to 2.2 ppb after treatment. In contrast, the 2016 maize sample, which had 1.8 ppb before treatment, reduced to 1.7 ppb; the 2017 sample had 1.9 ppb reduced to 0.9 ppb; and 2018 had 3 ppb before treatment reduced to 0.3 ppb after treatment; the AfB1 profiles depict that 2015 maize sample had 60 ppb before treatment and reduced to 1ppb after treatment. The 2016 maize sample had one ppb reduced to 0.9 ppb, whereas the 2017 sample had 1 ppb and reduced to 0.6 ppb, and 2018 had 2 ppb and detoxified to 0 ppb after treatment. The Bentonite clay used was found to reduce the toxin levels of the stored maize.

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References

Amadi, J.E., and Adeniyi, D.O., (2009). Mycotoxin production by fungi isolate from stored grains, African Journal of Biotechnology, 8, 1219-1221.

Benkerroum, N. (2020). Aflatoxins: Producing-molds, structure, health issues and incidence in Southeast Asian and Sub-Saharan African countries. International journal of environmental research and public health, 17(4), 1215. DOI: https://doi.org/10.3390/ijerph17041215

Bii, F., Wanyoike, W., Nyende, A.B., Gituru, R.W., and Bii, C., (2012): Fumonisin contamination of maize (Zea mays) in aflatoxin “hot” zones in Eastern province of Kenya. African Journal of Health Sciences, 20, (1-2) 28-36.

Blandino, M,, Reyneri, A., and Vanara, F.(2009). Effect of sowing time on toxigenic fungal infection and mycotoxin contamination of maize kernels. Journal of Phytopathology, 157, 7-14. DOI: https://doi.org/10.1111/j.1439-0434.2008.01431.x

Bouraima, Y., Ayi-Fianou, L., Kora. I., Sanni. A., and Creppy, E. E., (1993). Mise en evidencede la contamination des cereals par les aflatoxines et l’ochratoxine A au Benin. In Creppy, E. E., Castegnaro, M. and Dirheimer, G. (Eds) Human ochratoxicosis and its pathologies 231: 101-110.

Chulze, S.N., (2010) Strategies to reduce mycotoxin levels in maize during storage: a review Food Additives and Contaminants 27, 5, 651-657. DOI: https://doi.org/10.1080/19440040903573032

Eskola, M., Kos, G., Elliott, C. T., Hajšlová, J., Mayar, S., & Krska, R. (2020). Worldwide contamination of food-crops with mycotoxins: Validity of the widely cited ‘FAO estimate’of 25%. Critical reviews in food science and nutrition, 60(16), 2773-2789. DOI: https://doi.org/10.1080/10408398.2019.1658570

Felsentein, J., (1985) Confidence limits on phylogenes; An approach using the bootstraps, evolution 39; 783-791. DOI: https://doi.org/10.1111/j.1558-5646.1985.tb00420.x

Hell, K., Cardwell, K.F., Setamou, M., and Poehling, H., (2000). The Influence of storage practices on aflatoxin contamination in maize in four Agroecological zones of Benin, West Africa Journal of Stored Products Reserved 34(4): 1-2. DOI: https://doi.org/10.1016/S0022-474X(99)00056-9

Hell, K., Cardwell, K.F., and Poehling, H.M., (2003): Relationship between management practices, fungal infection and aflatoxin for stored maize in Benin. Journal of Phytopathology, 151, 690-698. DOI: https://doi.org/10.1046/j.1439-0434.2003.00792.x

Hillier, S. and Lumsden, D.,(2008) Distinguishing opaline silica from cristobalite in bentonites: a practical procedure and perspective based on NaOH dissolution, Clay Minerals, 43, 477- 486. DOI: https://doi.org/10.1180/claymin.2008.043.3.11

IARC.(1993). Aflatoxins: Naturally occurring aflatoxins (Group 1), aflatoxins M1 (Group 2B). International Agency Resource Cancer. 56:245.

Kpodo, K. A., (1996). Mycotoxins in maize and fermented maize products in Southern Ghana: In Proceedings of the workshop on mycotoxins in food in Africa. November 6 – 10, 1995 at Cotonou, Benin. International Institute of Tropical Agriculture, Benin, 33.

Krnjaja, V., Lević, J., Tomić, Z., Nešić, Z., Stojanović, L. J., and Trenkovski, S., (2007): Dynamics of incidence and frequency of populations of Fusarium species on stored maize grain. Biotechnology in Animal Husbandry, 23, 1, 5-6, 589-600. DOI: https://doi.org/10.2298/BAH0701589K

Miller, J.D.,(1993): The toxicological significance of mixtures of fungal toxins. African Newsletter on Occupational Health and Safety, 3 32-38.

McClure, R., Smith, C., and Dixon, J.B., (2014) Bentonite properties, formation, and distribution as adsorbents of aflatoxin in grain—The Texas case studies. In Aflatoxin Control: Safeguarding Animal Feed with Calcium Smectite; Dixon, 17 162 170.

Murphy, P.A., Hendrich, S., Landgren, C., and Bryant, C.M., (2006) Food mycotoxins: An update Journal of Food Science. 71: R51–R65. DOI: https://doi.org/10.1111/j.1750-3841.2006.00052.x

Olivier p., Amaranta C. C., Ama L. M., S. Tadrist, D. K. Akaki, R. K.Nevry, G. G. Moore, S. O. Fapohunda, S. Bailly, D. Montet, I. P. Oswald, S. Lorber, C. Barbet (2017). “Aspergillus korhogoensis, A Novel Aflatoxin producing species from the coted’voire”. Toxins 1-18.

Oyeleke, A. and Manga, S.B. (2008). Essential of Laboratory Practice, 3rd edition, Tobes Publishers, Minna, Niger State, Nigeria.12-29.

Remešova, J., Kolařík, M., and Prášil, K., (2007): Microfungi on the kernels of transgenic and non-transgenic maize damaged by the European corn borer. 59, 2, 205-213. DOI: https://doi.org/10.33585/cmy.59207

Saitou, N., and Nei, M., (1987) The neighbor joining method; A new method reconstructing phylogenetic tree. Journal of Molecular Biology and evolution 4 406-425.

Tamura, K., Nei, M., and Kumar, S., (2004) Prospect of inferring very large phylogenes by using the neighbor joining method, Proceedings of national academy of sciences (USA) 101; 11030-11035. DOI: https://doi.org/10.1073/pnas.0404206101

Temu, G. E. (2016). Molecular Identification of Aspergillus Strains and quick detection of aflatoxin from selected common spices in Tanzania. Journal of Scientific Research and Reports, 10(7), 1-8. DOI: https://doi.org/10.9734/JSRR/2016/26102

Tiffany, I., (2013). The implication of aflatoxin contamination for local food safety in Senegal. World Mycotoxin Journal 5, 225-256.

Tuite, J., Koh-knox, C., Stroshine, R., Cantone, F.A., and Bauman, L.F., (1985): Effect of physical damage to corn kernels on the development of Penicillium species and Aspergillus glaucus in storage. Postharvest Pathology and Mycotoxins, 75, 10, 1137-1140. DOI: https://doi.org/10.1094/Phyto-75-1137

Phillips, T.D., (1999) Dietary clay in the chemoprevention of aflatoxin-induced disease. Toxicological Science. 52, 118–126. DOI: https://doi.org/10.1093/toxsci/52.2.118

Sambrook, J., and Russel ,D.W., (2001). Molecular Cloning: A Laboratory Manual 3rd Edition. Cold Spring Harbor Laboratory Press. Cold Spring Harbor, New York. 69-78.

Tafinta I. Y., K. Shehu, H. Abdulganiyyu, A. M. Rabe and A. Usman (2013). Isolation and identification of fungi associated with spoilage of sweet oranges (citrus sinensis) fruits in Sokoto state. Nigerian journal of basic and applied science (3)21:193-196. DOI: https://doi.org/10.4314/njbas.v21i3.4

Usman, M., Javed, M. R., Mehmood, M. A., Huma, T., & Ijaz, A. (2019). Isolation of aflatoxigenic Aspergillus flavus from animal-feed and exploration of the genetic basis of aflatoxin biosynthesis. Pak. Vet. J.(in press). doi, 10. DOI: https://doi.org/10.29261/pakvetj/2019.078

Udoh, J. M., Cardwel, K. F., and Ikotun, T., (2000). Storage structures and aflatoxin content of maize in five agro-ecological zones of Nigeria. Journal of Stored Product. Reserved. 36: 187-201. DOI: https://doi.org/10.1016/S0022-474X(99)00042-9

Viegas, C., Sabino, R., and Viegas, S., (2015). Feed contamination by potential toxigenic fungi and of A. flavus complex. Toxicology Letters. (2) 238. DOI: https://doi.org/10.1016/j.toxlet.2015.08.255

Wang, J.H., Zhang, J.B., Chen, F.F., Li, H.P., Ndoye, M.,and Liao, Y.C.,(2012) A multiplex PCR assay for genetic chemotyping of toxigenic Fusarium graminearum and wheat grains for 3-acetyldeoxynivalenol and nivalenol mycotoxin. Journal of food and Agricultural environment.10, 505-511.

Whitlow, L.W., and Hagler, W.M., (2013). Mycotoxin contamination of feedstuffs – some additional stress factor Diary cattle, 32. www.cals.ncsu.edu/.../myco~1. pdf Retrieved November, 10, 2013.

Williams, J.H., Phillips, T.D., Jolly, P.E., Stiles, J.K., Jolly, C.M., and Aggarwal, D., (2004) Human aflatoxicosis in developing countries: A review of toxicology, exposure, potential health consequences, and interventions. American Journal of Clinical Nutrition DOI: https://doi.org/10.1093/ajcn/80.5.1106